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Fig. 2 <t>CircRNA</t> RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. *P < 0.05, **P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR
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Fig. 2 CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. *P < 0.05, **P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR

Journal: Journal of animal science and biotechnology

Article Title: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein.

doi: 10.1186/s40104-024-01003-w

Figure Lengend Snippet: Fig. 2 CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. *P < 0.05, **P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR

Article Snippet: CircRNA RNA-seq analysis on the same samples was performed by Novogene Technology Co., Ltd. (Beijing, China).

Techniques: Sequencing, RNA Sequencing, Expressing, Derivative Assay, Quantitative RT-PCR, Biomarker Discovery

CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR

Journal: Journal of Animal Science and Biotechnology

Article Title: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

doi: 10.1186/s40104-024-01003-w

Figure Lengend Snippet: CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR

Article Snippet: CircRNA RNA-seq analysis on the same samples was performed by Novogene Technology Co., Ltd. (Beijing, China).

Techniques: Sequencing, RNA Sequencing, Expressing, Derivative Assay, Quantitative RT-PCR, Biomarker Discovery

CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR

Journal: Journal of Animal Science and Biotechnology

Article Title: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

doi: 10.1186/s40104-024-01003-w

Figure Lengend Snippet: CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR

Article Snippet: A circRNA RNC-seq analysis was performed by Gene Denovo Biotechnology Co., Ltd. (Guangzhou, Guangdong, China).

Techniques: Sequencing, RNA Sequencing, Expressing, Derivative Assay, Quantitative RT-PCR, Biomarker Discovery